Journal: Journal of Hazardous Materials

Article Title: Increased survivability of coronavirus and H1N1 influenza virus under electrostatic aerosol-to-hydrosol sampling

doi: 10.1016/j.jhazmat.2021.125417

Figure Lengend Snippet: Schematic representation of the test with H1N1 influenza virus and HCoV-229E aerosols.

Article Snippet: Then, the cells in the 75- and 175-cm 2 cell-culture flasks were infected, respectively, using 3 and 6 mL FBS-antibiotic-antimycotic-free MEM/EBSS containing the H1N1 influenza virus (A/California/4/2009), initially obtained from BioNano Health-Guard Research Center (Daejeon, Korea), at a multiplicity of infection (MOI) of 0.01.

Techniques: Virus

Journal: Journal of Hazardous Materials

Article Title: Increased survivability of coronavirus and H1N1 influenza virus under electrostatic aerosol-to-hydrosol sampling

doi: 10.1016/j.jhazmat.2021.125417

Figure Lengend Snippet: qRT-PCR amplification curves with the prepared H1N1 influenza virus and HCoV-229E hydrosols after corona discharge exposure. (a) Test with the prepared H1N1 virus (DI). (b) Test with the prepared H1N1 virus (PBS+AA). (c) The effect of liquid flow rate (exposure time) on the H1N1 virus suspensions. (d) Test with the prepared HCoV-229E (DI). (e) Test with the prepared HCoV-229E (PBS+AA). (f) The effect of liquid flow rate (exposure time) on the HCoV-229E suspensions. The results represent the mean ± SEM of three independent assays. Significant differences are represented by * p < 0.05, ** p < 0.01, and *** p < 0.001. DI, deionized water; PBS+AA, phosphate-buffered saline and ascorbic acid solution; NE, non-exposed; RFU, relative fluorescence units.

Article Snippet: Then, the cells in the 75- and 175-cm 2 cell-culture flasks were infected, respectively, using 3 and 6 mL FBS-antibiotic-antimycotic-free MEM/EBSS containing the H1N1 influenza virus (A/California/4/2009), initially obtained from BioNano Health-Guard Research Center (Daejeon, Korea), at a multiplicity of infection (MOI) of 0.01.

Techniques: Quantitative RT-PCR, Amplification, Virus, Saline, Fluorescence

Journal: Journal of Hazardous Materials

Article Title: Increased survivability of coronavirus and H1N1 influenza virus under electrostatic aerosol-to-hydrosol sampling

doi: 10.1016/j.jhazmat.2021.125417

Figure Lengend Snippet: Comparison of H1N1-HA content and H1N1 influenza virus & HCoV-229E infectivity between the DI and PBS+AA hydrosols after corona discharge exposure. (a) H1N1-HA antibody quantification. (b) H1N1 influenza virus plaque titrations assay. (c) HCoV-229E plaque titrations assay. The results represent the mean ± SEM of three independent assays. Significant differences are represented by * p < 0.05, ** p < 0.01, and *** p < 0.001. DI, deionized water; PBS+AA, phosphate-buffered saline and ascorbic acid solution; NE, non-exposed.

Article Snippet: Then, the cells in the 75- and 175-cm 2 cell-culture flasks were infected, respectively, using 3 and 6 mL FBS-antibiotic-antimycotic-free MEM/EBSS containing the H1N1 influenza virus (A/California/4/2009), initially obtained from BioNano Health-Guard Research Center (Daejeon, Korea), at a multiplicity of infection (MOI) of 0.01.

Techniques: Comparison, Virus, Infection, Saline

Journal: Journal of Hazardous Materials

Article Title: Increased survivability of coronavirus and H1N1 influenza virus under electrostatic aerosol-to-hydrosol sampling

doi: 10.1016/j.jhazmat.2021.125417

Figure Lengend Snippet: Evaluation of the protective effect of PBS+AA using tests with H1N1 influenza virus and HCoV-229E aerosols. In HIN1 influenza virus: (a) The size distribution of the aerosolized virus. (b) qRT-PCR amplification curves for the DI and PBS+AA samples. (c) The effect of liquid type on the PCR C t values in DI and PBS+AA. (d) H1N1-HA antibody quantification. (e) Plaque titrations assay. In HCoV-229E: (f) The size distribution of the aerosolized virus. (g) qRT-PCR amplification curves for the DI and PBS+AA samples. (h) The effect of liquid type on the PCR Ct values in DI and PBS+AA. (i) Plaque titrations assay. (j) The correlation between PCR and virus viability results. The results represent the mean ± SEM of three independent assays. Significant differences are represented by * p < 0.05, ** p < 0.01, and *** p < 0.001. DI, deionized water; PBS+AA, phosphate-buffered saline and ascorbic acid solution; NE, non-exposed; RFU, relative fluorescence units.

Article Snippet: Then, the cells in the 75- and 175-cm 2 cell-culture flasks were infected, respectively, using 3 and 6 mL FBS-antibiotic-antimycotic-free MEM/EBSS containing the H1N1 influenza virus (A/California/4/2009), initially obtained from BioNano Health-Guard Research Center (Daejeon, Korea), at a multiplicity of infection (MOI) of 0.01.

Techniques: Virus, Quantitative RT-PCR, Amplification, Saline, Fluorescence